Difference between revisions of "Part:BBa K2273012:Design"
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===References===
 
===References===
  
−
[https://www.ncbi.nlm.nih.gov/pubmed/14762007 Susanna <i>et al.] <br>
+
Susanna KA, van der Werff AF, den Hengst CD, Calles B, Salas M, Venema G, Hamoen LW, Kuipers OP.(2014) <i>Mechanism of transcription activation at the comG promoter by the competence transcription factor ComK of Bacillus subtilis.</i> J Bacteriol. 2004 Feb;186(4):1120-8.[https://www.ncbi.nlm.nih.gov/pubmed/14762007 PubMed] <br>
 
Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br>
 
Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br>

Revision as of 15:10, 31 October 2017


PcomG: a ComK-dependent promoter of Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the rapA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The rapA promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PcomG fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAGAAACAGCATAATGAAAATGG
PcomG rev RFC10 gatcCTGCAGCGGCCGCTACTAGTATTCAACGCATATTGTAGAAAAAG

References

Susanna KA, van der Werff AF, den Hengst CD, Calles B, Salas M, Venema G, Hamoen LW, Kuipers OP.(2014) Mechanism of transcription activation at the comG promoter by the competence transcription factor ComK of Bacillus subtilis. J Bacteriol. 2004 Feb;186(4):1120-8.PubMed
Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed