Difference between revisions of "Part:BBa K2365041"
| Line 22: | Line 22: | ||
===[[File:TDH3.jpg|300px|center]]=== | ===[[File:TDH3.jpg|300px|center]]=== | ||
Figure1:TDH3 construct was transfected into Saccharomyces cerevisiae cells, the green fluorescence was observed under fluorescence microscope clearly. It indicates this part could work well. | Figure1:TDH3 construct was transfected into Saccharomyces cerevisiae cells, the green fluorescence was observed under fluorescence microscope clearly. It indicates this part could work well. | ||
| + | |||
Improved design: | Improved design: | ||
Revision as of 14:58, 31 October 2017
TDH3 promoter
a Constitutive promoter from Saccharomyces cerevisiae
Glyceraldehyde-3-phosphate dehydrogenase, -3- three glyceraldehyde dehydrogenase promoter (tdh1, tdh2, tdh3, tdh1) for weak promoter, tdh3 promoter, 3 phosphoglyceraldehyde catalytic oxidation (dehydrogenation) and phosphorylation of 1,3- - generation, is the central link of glucose metabolism, plays an important role in sugar metabolism.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Figure1:TDH3 construct was transfected into Saccharomyces cerevisiae cells, the green fluorescence was observed under fluorescence microscope clearly. It indicates this part could work well.
Improved design:
We construct the device： TDH3 promoter+GFP+ADH1 terminator. TDH3 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence.
Characterization：
Figure2:The responses of promoter strengths of artificial TDH3 promoter and native promoter TDH3 were comprehensively compared. The strength of improved TDH3 was always higher than native promoter TDH3，but varied along with the genetic background of host.