Difference between revisions of "Part:BBa K2371003:Design"

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<partinfo>BBa_K2371003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2371003 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The first two Biobricks are the two split T7 polymerase sequence we adopted. We provide them for other team to leverage them through other methods. We choose the split site suggested by the team of Tiyun Han, Quan Chen and Haiyan Liu.( Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.)
 
  
 
===Source===
 
===Source===
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Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.
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===References===
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1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216.
  
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2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.
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T7 polymerase from bacteria BL21(DE3)
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===References===
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Latest revision as of 14:44, 31 October 2017


C-T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Source

Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.

References

1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216.

2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.