Difference between revisions of "Part:BBa K2378005"
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This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoters BBa_J23106. | This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoters BBa_J23106. | ||
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| + | <p style="font-size: 18px"><strong>SEM (Scanning Electron Microscopy) Analysis</strong></p> | ||
<p>In order to observe the ability of our bacteria (transformed with BBa_K2378005) to degrade PET plastic, we ran a qualitative SEM analysis. PET plastic bottles were cut into 1x1 cm fragments with uniform weights. The plastic fragments were then washed with ethanol and water, followed by incubation in LB media contaning the bacterial transformants for 2 days. Following that, the plastic fragments were once again washed, dried, and finally observed under Scanning Electron Microscope (SEM). As the control, the plastic fragments were treated with bacterial cells without plasmid containing PETase. | <p>In order to observe the ability of our bacteria (transformed with BBa_K2378005) to degrade PET plastic, we ran a qualitative SEM analysis. PET plastic bottles were cut into 1x1 cm fragments with uniform weights. The plastic fragments were then washed with ethanol and water, followed by incubation in LB media contaning the bacterial transformants for 2 days. Following that, the plastic fragments were once again washed, dried, and finally observed under Scanning Electron Microscope (SEM). As the control, the plastic fragments were treated with bacterial cells without plasmid containing PETase. | ||
SEM results show significantly rougher surface with more cracks in samples treated with BBa_K2378005 transformants compared to its control. We can thus conclude that BBa_K2378005 works as intended in biodegrading PET plastics.</p> | SEM results show significantly rougher surface with more cracks in samples treated with BBa_K2378005 transformants compared to its control. We can thus conclude that BBa_K2378005 works as intended in biodegrading PET plastics.</p> | ||
Revision as of 14:37, 31 October 2017
Constitutive PETase Coding Device
This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoters BBa_J23106.
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SEM (Scanning Electron Microscopy) Analysis
In order to observe the ability of our bacteria (transformed with BBa_K2378005) to degrade PET plastic, we ran a qualitative SEM analysis. PET plastic bottles were cut into 1x1 cm fragments with uniform weights. The plastic fragments were then washed with ethanol and water, followed by incubation in LB media contaning the bacterial transformants for 2 days. Following that, the plastic fragments were once again washed, dried, and finally observed under Scanning Electron Microscope (SEM). As the control, the plastic fragments were treated with bacterial cells without plasmid containing PETase. SEM results show significantly rougher surface with more cracks in samples treated with BBa_K2378005 transformants compared to its control. We can thus conclude that BBa_K2378005 works as intended in biodegrading PET plastics.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 70
Illegal NotI site found at 979 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 724
- 1000COMPATIBLE WITH RFC[1000]