Difference between revisions of "Part:BBa K2365041"
Line 20: Line 20:
 
===[[File:TDH3.jpg|300px|center]]===
 
===[[File:TDH3.jpg|300px|center]]===
 
Improved design:
 
Improved design:
 +
 
We construct the device：
 
We construct the device：
 
TDH3 promoter+GFP+ADH1 terminator
 
TDH3 promoter+GFP+ADH1 terminator
 +
 
TDH3 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence.
 
TDH3 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence.
 +
 +
Characterization：
 
===[[File:FIGURE .2.png|300px|center]]===
 
===[[File:FIGURE .2.png|300px|center]]===
 
Figure2:The responses of promoter strengths of artificial TDH3 promoter and native promoter TDH3 were comprehensively compared. The strength of improved TDH3 was always higher than native promoter TDH3，but varied along with the genetic background of host.
 
Figure2:The responses of promoter strengths of artificial TDH3 promoter and native promoter TDH3 were comprehensively compared. The strength of improved TDH3 was always higher than native promoter TDH3，but varied along with the genetic background of host.

Revision as of 14:22, 31 October 2017


TDH3 promoter

a Constitutive promoter from Saccharomyces cerevisiae

Glyceraldehyde-3-phosphate dehydrogenase, -3- three glyceraldehyde dehydrogenase promoter (tdh1, tdh2, tdh3, tdh1) for weak promoter, tdh3 promoter, 3 phosphoglyceraldehyde catalytic oxidation (dehydrogenation) and phosphorylation of 1,3- - generation, is the central link of glucose metabolism, plays an important role in sugar metabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


TDH3.jpg

Improved design:

We construct the device： TDH3 promoter+GFP+ADH1 terminator

TDH3 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence.

Characterization：

FIGURE .2.png

Figure2:The responses of promoter strengths of artificial TDH3 promoter and native promoter TDH3 were comprehensively compared. The strength of improved TDH3 was always higher than native promoter TDH3，but varied along with the genetic background of host.