Difference between revisions of "Part:BBa K2273002:Design"

 
 
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===Design Notes===
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===Design===
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tba
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This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>rapF</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
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===Source===
 
===Source===
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The <i>rapF</i> promoter was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
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<br>
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<table>
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<tr>
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<td width="150">PrapF fwd RFC10</td>
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<td>gatcGAATTCGCGGCCGCTTCTAGAGAAACATGCGGTGTAACGAAAG</td>
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</tr>
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<tr>
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<td>PrapF rev RFC10</td>
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<td>gatcCTGCAGCGGCCGCTACTAGTATCATAATTCTATATATGTCATATTTTATC</td>
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</tr>
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</table>
  
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gDNA of <i>Bacillus subtilis</i>
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===References===
  
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===References===
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Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) <i>The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs.</i> Nucleic Acids Res.44(5):2160-72.
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[https://www.ncbi.nlm.nih.gov/pubmed/?term=The%20quorum-sensing%20regulator%20ComA%20from%20Bacillus%20subtilis%20activates%20transcription%20using%20topologically%20distinct%20DNA%20motifs PubMed]<br><br>
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Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br>

Latest revision as of 14:14, 31 October 2017


PrapF: a ComA-dependent promoter of Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the rapF promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The rapF promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PrapF fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAGAAACATGCGGTGTAACGAAAG
PrapF rev RFC10 gatcCTGCAGCGGCCGCTACTAGTATCATAATTCTATATATGTCATATTTTATC

References

Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res.44(5):2160-72. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed