Difference between revisions of "Part:BBa K2243002"
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Through the random mutation from iGEM registry RBS, one well-behaved RBS D4 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct. | Through the random mutation from iGEM registry RBS, one well-behaved RBS D4 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct. | ||
− | + | ||
− | ===Usage | + | ===Usage:=== |
+ | D4_Bxb1 gp35 is one of the best expression system. We added RBS D4 upstream the Bxb1 gp35 coding sequence (BBa_K22430012). | ||
+ | |||
+ | ===Biology:=== | ||
+ | The Bxb1 coding sequence is the part BBa_K22430012. | ||
+ | |||
+ | ===Design note:=== | ||
+ | We construct this structure by Gibson Assembly. The D4 RBS is generate by random mutation. | ||
+ | |||
+ | ===Characterize:=== | ||
+ | We characterize the D4_Bxb1 gp35 in a novel structure, which composed with expressional and transcriptional regulatory elements, and with a corresponding reporter system (BBa_K2243006). | ||
+ | |||
+ | |||
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Revision as of 14:08, 31 October 2017
D4_Bxb1 gp35
Through the random mutation from iGEM registry RBS, one well-behaved RBS D4 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.
Usage:
D4_Bxb1 gp35 is one of the best expression system. We added RBS D4 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).
Biology:
The Bxb1 coding sequence is the part BBa_K22430012.
Design note:
We construct this structure by Gibson Assembly. The D4 RBS is generate by random mutation.
Characterize:
We characterize the D4_Bxb1 gp35 in a novel structure, which composed with expressional and transcriptional regulatory elements, and with a corresponding reporter system (BBa_K2243006).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 192
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 466
Illegal XhoI site found at 553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1105
Illegal NgoMIV site found at 1192
Illegal AgeI site found at 242 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1300