Difference between revisions of "Part:BBa K2365035"

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====PCR and SDS-PAGE====
 
====PCR and SDS-PAGE====
 
Showing the size of the target gene.
 
Showing the size of the target gene.
 +
 
[[File:ZHD101 DNA.jpg|400px]]
 
[[File:ZHD101 DNA.jpg|400px]]
 
[[File:ZHD101 SDS.jpg|400px]]
 
[[File:ZHD101 SDS.jpg|400px]]

Revision as of 12:57, 31 October 2017


ZHD101--Zearalenone degrading enzyme (optimized)

the gene encoding ZHD101(a ZEN-degrading enzyme)was cloned. ZHD101, a lactonohydrolase produced by the fungal species Clonostachys rosea, converts ZEN into 1-(3,5-dihydroxy-phenyl)-10-hydroxy-1-undecen- 6-one, which is a markedly less toxic product.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


PCR and SDS-PAGE

Showing the size of the target gene.

ZHD101 DNA.jpg ZHD101 SDS.jpg