Difference between revisions of "Part:BBa K2365511"
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[[File:Cerope A 1.jpg|300px]] | [[File:Cerope A 1.jpg|300px]] | ||
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[[File:Cecropin A zone.jpeg|500px|center]] | [[File:Cecropin A zone.jpeg|500px|center]] | ||
[[File:Cecropin_A_595nm.png|600px|left]] | [[File:Cecropin_A_595nm.png|600px|left]] | ||
Revision as of 12:03, 31 October 2017
cecropinA
BBa_K2365511 encodes Cecropin A ,a naturally occurring,linear,cationic,antimicrobial peptide,it can inhibit the growth of Fusarium graminearum.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 14
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 106
Illegal AgeI site found at 144 - 1000COMPATIBLE WITH RFC[1000]
3-dimension structure
CecropinA (CA)is a naturally occurring, linear, cationic, 37-residue antimicrobial peptide. It has inhibitory effect on Aspergillus and Fusarium. The possible mechanism of killing bacteria is make the cell to form holes. This is the three-dimensional structure. It has the alpha helix and beta folding structure.
We did PCR and agarose gel electrophoresis to test and verify cecropinA. And then we did Tris tricine-SDS-PAGE to confirm if he successfully expressed. It’s has 177 bases and the kDa is about 5.The results show cecropinA was constructed and expressed successful.
