Difference between revisions of "Part:BBa K2365037"
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[[File:DON degrading enzyme SDS.jpg|400px]] | [[File:DON degrading enzyme SDS.jpg|400px]] | ||
| + | In theory, the higher one of the two absorption peaks for BL21+DON group and DON group should be the same. In fact, the result in the figure qualifies our inference.(dont in view of the extraction efficiency).Compared with our control groups(BL21+DON and DON), there is a peak valley in DON’s absorption peak for our test group BL21+DON+IPTG, from which the DON degrading enzyme can be induced and then expressed. It proves that the degrading enzyme expressed in BL21 has a significant degrading effect. | ||
[[Image:Uv_don.png||thumb|center|500px|Fig3 shows that we use the MSD to analyze the degrading effects of our DON degrading enzyme]] | [[Image:Uv_don.png||thumb|center|500px|Fig3 shows that we use the MSD to analyze the degrading effects of our DON degrading enzyme]] | ||
Revision as of 11:50, 31 October 2017
Trichothecene 3-O-acetyltransferase(AYT1)，an indispensable enzyme for the biosynthesis of trichothecenes, to catalyzes the transfer of an acetyl group from acetyl coenzyme A to the C3 hydroxyl moiety which will result in a switch from DON to 3ADON, so the toxicity will be reduced by 100-fold.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 213
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 306
Illegal BglII site found at 318
Illegal XhoI site found at 884 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PCR and SDS-PAGE
Showing the size of the target gene.
In theory, the higher one of the two absorption peaks for BL21+DON group and DON group should be the same. In fact, the result in the figure qualifies our inference.(dont in view of the extraction efficiency).Compared with our control groups(BL21+DON and DON), there is a peak valley in DON’s absorption peak for our test group BL21+DON+IPTG, from which the DON degrading enzyme can be induced and then expressed. It proves that the degrading enzyme expressed in BL21 has a significant degrading effect.
