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| − | __NOTOC__
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| − | <partinfo>BBa_K2507014 short</partinfo>
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| − | ==Usage and Biology==
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| − | E.coli codon optimized TtrS(BBa_K2507002) and TtrR(BBa_K2507003) are two basic parts which belong to the two-component system from marine Shewanella baltica. TtrS is the membrane-bound sensor kinase(SK) which can sense tetrathionate outside the cell and TtrR is the DNA-binding response regulator(RR).PttrB185-269 (BBa_K2507019) is a minimal TtrR activated promoter when TtrR is phosphorylated by TtrS after TtrS sensing tetrathionate.
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| − | Winter et.al have shown that reactive oxygen species (ROS) produced by the host during inflammation convert thiosulfate to tetrathionate, which this pathogen consumes to establish a startpoint for infection (Winter et al, 2010).Thus, tetrathionate may correlate with pro-inflammation conditions and can be used as gut inflammation sensor.
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| − | ===Usage and Biology===
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K2507014 SequenceAndFeatures</partinfo>
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| − | ==Characterization==
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| − | After validate this system in laboratory Escherichia coli Top10 and E.coli Nissle 1917, this system can function as a tetrathionate sensor and reporter.
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| − | [[File: SHSBNU 17 40a11.jpg|200px|thumb|left|alt text]]
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| − | Figure 1. Schematic of ligand-induced signaling through TtrS/R and plasmid design of the sensor components. TtrS/R were tested under the situation BBa_K2507006 was in pSB4K5 backbone and BBa_K2507014 was in pSB1C3 backbone. We submitted the parts all to the iGEM registry in pSB1C3.
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| − | ==Reference==
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| − | <p>
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| − | Daeffler, K. N., Galley, J. D., Sheth, R. U., Ortiz‐Velez, L. C., Bibb, C. O., & Shroyer, N. F., et al. (2017). Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Molecular Systems Biology, 13(4), 923.
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| − | </p>
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K2507014 parameters</partinfo>
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