Difference between revisions of "Part:BBa K2404022"

 
 
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<partinfo>BBa_K2404022 short</partinfo>
 
<partinfo>BBa_K2404022 short</partinfo>
  
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A thyroid stimulating hormone antagonist, with His-tags for purification, under control of the salicylic acid-inducible promoter, GST6. The terminator is the nopaline synthase gene terminator, native to <i> Agrobacterium tumefaciens </i>. This construct in contained within the pGB-A2 plasmid. It should be used to express and create the TSH antagonist in the presence of salicylic acid.
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This construct contains the [https://parts.igem.org/Part:BBa_K2404004 GST6 promotor], the [https://parts.igem.org/Part:BBa_K2404001 TSHH] coding sequence. We have not fully sequenced the 3' region of the construct but our PCR analysis indicates that it correctly includes the [https://parts.igem.org/Part:BBa_P10401 Nos Terminator].
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<br>
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This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10503 pGB-A2 plasmid].
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<br>
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The GST6 promoter is induced by the plant hormone salicylic acid..<br>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<br>
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1-6: BsmBI<br>
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16-513: PR2<br>
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514-1326: TSHH<br>
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1327-1593: NosT<br>
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1603-1608: BsmBI<br>
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<partinfo>BBa_K2404022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404022 SequenceAndFeatures</partinfo>
  

Latest revision as of 11:27, 31 October 2017


TSH antagonist under control of the GST6 promoter

This construct contains the GST6 promotor, the TSHH coding sequence. We have not fully sequenced the 3' region of the construct but our PCR analysis indicates that it correctly includes the Nos Terminator.


This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The GST6 promoter is induced by the plant hormone salicylic acid..


Sequence and Features
1-6: BsmBI
16-513: PR2
514-1326: TSHH
1327-1593: NosT
1603-1608: BsmBI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 72
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1300
    Illegal XhoI site found at 1112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 72
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 72
    Illegal PstI site found at 1045
    Illegal PstI site found at 1087
  • 1000
    COMPATIBLE WITH RFC[1000]