Difference between revisions of "Part:BBa K2404018"
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<partinfo>BBa_K2404018 short</partinfo>
 
<partinfo>BBa_K2404018 short</partinfo>
  
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A thyroid stimulating hormone antagonist, with His-tags for purification, under control of the jasmonic acid-inducible promoter, PDF1.2. The terminator is the nopaline synthase gene terminator, native to <i> Agrobacterium tumefaciens </i>. This construct in contained within the pGB-A2 plasmid. It should be used to express and create the TSH antagonist in the presence of jasmonic acid.
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This construct contains the [https://parts.igem.org/Part:BBa_K2404002 PDF1.2 promotor], and the [https://parts.igem.org/Part:BBa_K2404001 TSHH] coding sequence. This does not contain a terminator sequence.
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<br>
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This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10503 pGB-A2 plasmid].
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<br>
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The PDF1.2 promoter is induced by the plant hormone jasmonic acid.<br>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>Sequence and Features</span><br>
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1-6: BsmBI<br>
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16-516: PDF1.2<br>
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517-1329: TSHH<br>
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1334-1339: BsmBI<br>
 
<partinfo>BBa_K2404018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404018 SequenceAndFeatures</partinfo>
  

Revision as of 10:37, 31 October 2017


TSH antagonist under control of the PDF1.2 promoter

This construct contains the PDF1.2 promotor, and the TSHH coding sequence. This does not contain a terminator sequence.
This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The PDF1.2 promoter is induced by the plant hormone jasmonic acid.


Sequence and Features
1-6: BsmBI
16-516: PDF1.2
517-1329: TSHH
1334-1339: BsmBI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 412
    Illegal BamHI site found at 1303
    Illegal XhoI site found at 1115
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 1000
    COMPATIBLE WITH RFC[1000]