Difference between revisions of "Part:BBa K2271066:Design"

Latest revision as of 10:33, 31 October 2017

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 860
Illegal BamHI site found at 1577
Illegal XhoI site found at 1613
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The part was designed as a marker for the peroxisomal lumen.

Source

The Promoter, mRuby and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee et al. (2015). [2]
ePts1 was synthesized by IDT.

References

[1] Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways
William C. DeLoache, Zachary N. Russ & John E. Dueber
Nat. Commun. 7:11152 doi: 10.1038/ncomms11152 (2016).

[2] A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly (2015)
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v

@@ Line 13: / Line 13: @@
 ===References===
+[1] <b>Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways </b> <br>
+William C. DeLoache, Zachary N. Russ & John E. Dueber <br>
+Nat. Commun. 7:11152 doi: 10.1038/ncomms11152 (2016).
 [2] <b>A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly </b> (2015)  <br>
 Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber <br>
 ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v