Difference between revisions of "Part:BBa K2271066:Design"
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===Source===
 
===Source===
  
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The Promoter, mRuby and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee <i>et al.</i> (2015). [1]
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The Promoter, mRuby and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee <i>et al.</i> (2015). [2]
 
<br>
 
<br>
 
ePts1 was synthesized by IDT.
 
ePts1 was synthesized by IDT.
  
 
===References===
 
===References===
 +
[1] <b>Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways </b> <br>
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William C. DeLoache, Zachary N. Russ & John E. Dueber <br>
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Nat. Commun. 7:11152 doi: 10.1038/ncomms11152 (2016).
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 +
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[2] <b>A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly </b> (2015)  <br>
 +
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber <br>
 +
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v

Latest revision as of 10:33, 31 October 2017

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
    Illegal BamHI site found at 1577
    Illegal XhoI site found at 1613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The part was designed as a marker for the peroxisomal lumen.

Source

The Promoter, mRuby and Terminator were obtained from A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly from Lee et al. (2015). [2]
ePts1 was synthesized by IDT.

References

[1] Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways
William C. DeLoache, Zachary N. Russ & John E. Dueber
Nat. Commun. 7:11152 doi: 10.1038/ncomms11152 (2016).


[2] A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly (2015)
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v