Difference between revisions of "Part:BBa K2505002"
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<p>This gene is derived from <i>Arabidopsis thaliana</i> and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones)   
 
<p>This gene is derived from <i>Arabidopsis thaliana</i> and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones)   
−
in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112:   
+
in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase ('''[EC:2.5.1.112]''':   
 
cytokinin synthase) activity and catalyzes the transfer of an   
 
cytokinin synthase) activity and catalyzes the transfer of an   
 
isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP,   
 
isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP,   

Revision as of 10:32, 31 October 2017

atipt4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 881


This gene is derived from Arabidopsis thaliana and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase ([EC:2.5.1.112]: cytokinin synthase) activity and catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, producing cytokinin nucleotides. Note that cytokinin nucleotides are the immature form.

The DNA sequences of these genes are optimized for expressing in human cells considering the codon usage.


Characterization

We tested the expression of AtIPT4 protein in human cell(EA.hy626) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin).