Difference between revisions of "Part:BBa K2206013"

Latest revision as of 10:22, 31 October 2017

Anti-miRNA for hsa-miR-15b-5p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch. The formation of the duplex occurs through a highly specific binding step which results in single-base mismatch specificity.

The trigger RNA sequence for BBa_K2206012 is divided between hsa-miR-15b-5p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206012. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-15b-5p and one which is complimentary to the 5' end of BBa_K2206012.

For more information, see the section on second series of toehold switches on the design page of CLSB-UK's 2017 wiki - http://2017.igem.org/Team:CLSB-UK/Design

NUPACK Structure Analysis

Sequence and Features