Difference between revisions of "Part:BBa K2271066"

Revision as of 10:10, 31 October 2017

mRuby-ePTS1

Usage and Biology

This Part is a composite Part containing the fluorescent protein mRuby targeted to the peroxisome with an enhanced PTS1 (Dueber et al.). The part is designed as a peroxisomal lumen marker for S. cerevisiea. Using the TDH3 promoter and the HHF1 terminator. For fluorometric and microscopic applications the optimal excitation of 559 nm and emission of 600 nm is discriped.

Experimental Design and Results

For the validation the S. cerevisiae Strain BY4742 was tranformed with this part. The cells were fixated and microscoped with an Elyra PS microscope. A typical peroxisomal localisation could be validated (Figure 1).

Figure 1 mRuby fused to the ePts1 described by DeLoache et al. (2016) [1.] The mRuby is localised in the peroxisomes of the cells

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 860
Illegal BamHI site found at 1577
Illegal XhoI site found at 1613
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 === Experimental Design and Results===
 <p> For the validation the <i>S. cerevisiae</i> Strain BY4742 was tranformed with this part. The cells were fixated and microscoped with an Elyra PS microscope. A typical peroxisomal localisation could be validated (Figure 1).
-[[File:Igemducgn2017mRuby-PTS1.png|500px|thumb|center|'''Figure 1''' mRuby fused to the ePts1 described in DeLoache et al. (2016) [https://parts.igem.org/Part:BBa_K2271066:Design [1].] The mRuby is localised in the peroxisomes of the cells]] </p>
+[[File:Igemducgn2017mRuby-PTS1.png|500px|thumb|center|'''Figure 1''' mRuby fused to the ePts1 described by DeLoache et al. (2016) [https://parts.igem.org/Part:BBa_K2271066:Design [1].] The mRuby is localised in the peroxisomes of the cells]] </p>