Difference between revisions of "Part:BBa K2505002"
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| − | <p>This gene is derived from <i> | + | <p>This gene is derived from <i>Arabidopsis thaliana</i> and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones) |
in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112: | in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112: | ||
cytokinin synthase) activity and catalyzes the transfer of an | cytokinin synthase) activity and catalyzes the transfer of an | ||
Revision as of 10:03, 31 October 2017
atipt4
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 739
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 881
This gene is derived from Arabidopsis thaliana and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112: cytokinin synthase) activity and catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, producing cytokinin nucleotides. Note that cytokinin nucleotides are the immature form.
The DNA sequences of these genes are optimized for expressing in human cells considering the codon usage.
Contents
Characterization
We tested the expression of AtIPT4 protein in human cell(EA.hy626) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin).