Difference between revisions of "Part:BBa K2404014"

 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2404014 short</partinfo>
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<partinfo>BBa_K2404014 short</partinfo><br>
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We generated a luciferase construct to test the efficacy of the tobacco leave expression system.
  
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The luciferase firefly gene under control of the inducible promoter of the LexA gene, native to <i> E. coli </i>. This promoter is induced by XVE. This construct has the terminator from the Nopaline synthase gene, native to <i> Agrobacterium tumefaciens </i>. This construct is contained within the pGB-A2 plasmid. It should be used as an a construct to test the promoter strength of the LexA promoter, both with and without the presence of XVE.
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This construct contains the [https://parts.igem.org/Part:BBa_P10000 LexA promotor], the luciferase coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].
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This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10501 pGB-A1 plasmid].
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<br>
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The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.  
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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Sequence detail:<br>:<br>
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1-6: BsmBI:<br>
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7-348: LexA:<br>
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349-2005: LUC+:<br>
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2006-2272: NosT:<br>
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2282-2287: BsmBI:<br>
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<partinfo>BBa_K2404014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404014 SequenceAndFeatures</partinfo>
  

Latest revision as of 08:54, 31 October 2017


Luc+ gene under control of the LexA promoter
We generated a luciferase construct to test the efficacy of the tobacco leave expression system.

This construct contains the LexA promotor, the luciferase coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A1 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.

Sequence and Features Sequence detail:
:
1-6: BsmBI:
7-348: LexA:
349-2005: LUC+:
2006-2272: NosT:
2282-2287: BsmBI:


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1171
    Illegal BamHI site found at 1017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]