Difference between revisions of "Part:BBa K328003"

Latest revision as of 08:51, 31 October 2017

cspA Cold box + UTR + SD +DB

This is Cold Box + UTR + SD + DB to test it with other promoters and measure them. More info in BBa_K328001

Warning!. The use of an Dowstream Box sequence in a coding device could lead the cell to a state of a "low temperature antibiotic effect (LACE)" by truncated RNA with the DB sequence. For more info see:

Jiang,W. ,Hou,Y. and Inouye,M. (1997)CspA, themajor cold shock protein of Escherichia coli, is an RNA chaperone. J. Biol. Chem. 272, 196–202.

This part was improved upon as https://parts.igem.org/Part:BBa_K2282011 where it was used for conditional expression of an amilCP reporter only at low temperature (~15 C).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K328003 short</partinfo>
-This is Cold Box + UTR + SD + DB to test it with other promoters and measure them.
+This is Cold Box + UTR + SD + DB to test it with other promoters and measure them. More info in [[Part:BBa_K328001 | BBa_K328001]]
+Warning!. The use of an Dowstream Box sequence in a coding device could lead the cell to a state of a "low temperature antibiotic effect (LACE)" by truncated RNA with the DB sequence. For more info see:
+Jiang,W. ,Hou,Y. and Inouye,M. (1997)CspA, themajor cold shock protein of Escherichia coli, is an RNA chaperone. J.
+Biol. Chem. 272, 196–202.
+This part was improved upon as https://parts.igem.org/Part:BBa_K2282011 where it was used for conditional expression of an amilCP reporter only at low temperature (~15 C).
 <!-- Add more about the biology of this part here