Difference between revisions of "Part:BBa K2505030"
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This part constitutively produces C8. We introduced point mutation to train gene, the productivity of C8 was improved by 100-fold. We introduced this part to E. coli then E. coli could produced enough C8 to induce transcription of human cells.
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This part constitutively produces C8. We introduced point mutation to <i>traI</i> gene, the productivity of C8 was improved by x fold. We introduced this part to <i>E. coli</i> then <i>E. coli</i> could produced enough C8 to induce transcription of human cells.
  
  

Revision as of 08:50, 31 October 2017

Ptet-rbs-traI (K34G)-tt

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This part constitutively produces C8. We introduced point mutation to traI gene, the productivity of C8 was improved by x fold. We introduced this part to E. coli then E. coli could produced enough C8 to induce transcription of human cells.


Characterization and improvement

Result

Discussion

Material and Method