Difference between revisions of "Part:BBa K2250002"

Latest revision as of 03:10, 31 October 2017

RafD, an invertase followed by HlyA, a secretion signal

It is the coding sequence of RafD followed by a secretion signal of HlyA. And a GGGGS linker is between them. RafD is a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacteria can use sucrose in the medium. Signal peptide of HlyA can make it possible for RafD to be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein. GGGGS linker is added between RafD coding sequence and signal peptide of HlyA so that adding of signal peptide will not influence the folding and function of RafD. The secretion of RafD also needs help of a secretion system, which can be seen in the main page of part BBa_K1166002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1356
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 97
Illegal NgoMIV site found at 968
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 799

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 <partinfo>BBa_K2250002 short</partinfo>
-It is the coding sequence of RafD followed by a secretion signal HlyA. And a GGGGS linker is between them. RafD is a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacterial can use sucrose in the medium. HlyA tag can make it possible for RafD to be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein. GGGGS linker is added between RafD coding sequence and HlyA signal peptide so that adding of HlyA will not influence the folding and function of RafD. The secretion of RafD also needs help of a secretion system, which can be seen in the main page of part BBa_K1166002.
+It is the coding sequence of RafD followed by a secretion signal of HlyA. And a GGGGS linker is between them. RafD is a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacteria can use sucrose in the medium. Signal peptide of HlyA can make it possible for RafD to be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein. GGGGS linker is added between RafD coding sequence and signal peptide of HlyA so that adding of signal peptide will not influence the folding and function of RafD. The secretion of RafD also needs help of a secretion system, which can be seen in the main page of part BBa_K1166002.
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