Difference between revisions of "Part:BBa K2440030"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker. | ||
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| + | Dre-miR-152 is a novel miRNA founded in zebrafish.1 | ||
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| + | It was found that miR-152 was underexpressed in human osteosarcoma (OS) tissues and cell lines. Decreased miR-152 was inversely correlated with lymph-node metastasis and advanced clinical stage. Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration and invasion of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), as further confirmed by a dual-luciferase reporter assay. Dre-miR-152 may be a therapeutic target for OS2. | ||
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| + | Moreover, MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation3. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 01:55, 31 October 2017
miR-152 target sequence
It is the target sequence of miR-152, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.
Dre-miR-152 is a novel miRNA founded in zebrafish.1
It was found that miR-152 was underexpressed in human osteosarcoma (OS) tissues and cell lines. Decreased miR-152 was inversely correlated with lymph-node metastasis and advanced clinical stage. Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration and invasion of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), as further confirmed by a dual-luciferase reporter assay. Dre-miR-152 may be a therapeutic target for OS2.
Moreover, MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation3.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.