Difference between revisions of "Part:BBa K2440016"
(→‎Usage and Biology)
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.
 +
 +
Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.1
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It has been reported that hsa-miR-511-5p can act as an intracellular mediator of glucocorticoids and TGF-β for the induction of endotoxin tolerance in human monocytes2.
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 +
Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL patients3.
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 01:03, 31 October 2017


miR-511 target sequence

It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.

Hsa-miR-511-5p was firstly found in human placenta by an integrative approach combining bioinformatics predictions with microarray analysis and sequence-directed cloning. It is located in chr 10 17927113-17927199 and chr 10 18174042-18174128.1

It has been reported that hsa-miR-511-5p can act as an intracellular mediator of glucocorticoids and TGF-β for the induction of endotoxin tolerance in human monocytes2.

Decreased expression of miR-138-5p and increased expression of miR-147a, miR-147b and miR-511-5p in HCV DLBCL was found to be a poor prognostic factor for HCV-positive DLBCL patients3.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.