Difference between revisions of "Part:BBa K2505007"
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This part produces mCherry induced by some stress like osmotic pressure or low temperature.The genes(<i>cps</i>) are transcriptionally regulated by numerous proteins: RcsB, RcsC and AHK4.
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This is a promoter derived from <i>E. coli</i> and acativated through histidine-to-aspartate phosphorelay. A His-to-Asp phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. One of the His-to-Asp phosphorelay systems in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, and RcsB, a response regulator. This system is activated after stress exposure such as osmolality shock; cps operon promoter (which controls the production of polysaccharides) is induced through the RcsC→RcsD→RscB→cps pathway.
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Revision as of 01:01, 31 October 2017

Pcps-rbs-mCherry

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This is a promoter derived from E. coli and acativated through histidine-to-aspartate phosphorelay. A His-to-Asp phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. One of the His-to-Asp phosphorelay systems in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, and RcsB, a response regulator. This system is activated after stress exposure such as osmolality shock; cps operon promoter (which controls the production of polysaccharides) is induced through the RcsC→RcsD→RscB→cps pathway.

Characterization

Materials & Methods