Difference between revisions of "Part:BBa K2206000:Design"

(Design Notes)
 
(One intermediate revision by the same user not shown)
Line 9: Line 9:
 
There must be no stop codons between the start codon and the protein coding sequence.  
 
There must be no stop codons between the start codon and the protein coding sequence.  
  
The RBS and start codon should not be paired to anything.  
+
The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.
  
 
The distance between the start codon and the protein coding region has to be a multiple of three.
 
The distance between the start codon and the protein coding region has to be a multiple of three.
  
For more detail visit: http://2017.igem.org/Team:CLSB-UK
+
For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design
  
 
===Source===
 
===Source===

Latest revision as of 22:07, 30 October 2017


Toehold switch for hsa-miR-15b-5p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There must be no stop codons between the start codon and the protein coding sequence.

The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References