Difference between revisions of "Part:BBa K2206000:Design"

 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
We ensured that there are no stop codons between the start codon and the protein coding sequence.  
+
There must be no stop codons between the start codon and the protein coding sequence.  
  
The RBS and start codon should not be paired to anything.  
+
The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.
  
 
The distance between the start codon and the protein coding region has to be a multiple of three.
 
The distance between the start codon and the protein coding region has to be a multiple of three.
  
For more detail visit: http://2017.igem.org/Team:CLSB-UK  
+
For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design
  
 
===Source===
 
===Source===

Latest revision as of 22:07, 30 October 2017


Toehold switch for hsa-miR-15b-5p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There must be no stop codons between the start codon and the protein coding sequence.

The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References