Difference between revisions of "Part:BBa K2348000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The alx regulatory part contains the constitutiv promoter and the riboswitch itself | + | The alx regulatory part contains the constitutiv alx promoter and the riboswitch itself |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | <sup>1</sup>Bingham, R. J., Hall, K. S. & Slonczewski, J. L. Alkaline induction of a novel gene locus, alx, in | ||
| + | Escherichia coli. Journal of bacteriology 172, 2184–2186 (1990). | ||
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| + | <sup>2</sup>Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A., Westhof, E. & Altuvia, S. A pH-responsive | ||
| + | riboregulator. Genes & development 23, 2650–2662 (2009). | ||
Latest revision as of 21:54, 30 October 2017
alx - alkaline regulated riboswitch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 312
Design Notes
The alx regulatory part contains the constitutiv alx promoter and the riboswitch itself
Source
Colony PCR from E. coli BL21
References
1Bingham, R. J., Hall, K. S. & Slonczewski, J. L. Alkaline induction of a novel gene locus, alx, in Escherichia coli. Journal of bacteriology 172, 2184–2186 (1990).
2Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A., Westhof, E. & Altuvia, S. A pH-responsive riboregulator. Genes & development 23, 2650–2662 (2009).