Difference between revisions of "Part:BBa K2443037"
Line 4: | Line 4: | ||
T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator. | T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator. | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
<!-- Add more about the biology of this part here> | <!-- Add more about the biology of this part here> |
Revision as of 21:50, 30 October 2017
T7 RNA Polymerase optimized for expression in E. coli
T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in Escherichia coli and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.
Sequence and Features</p>
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1719
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 275
Illegal NgoMIV site found at 1496
Illegal NgoMIV site found at 1943 - 1000COMPATIBLE WITH RFC[1000]