Difference between revisions of "Part:BBa K2443037"

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T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.  
 
T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.  
 
<h1> Improved part: </h1>
 
<p class="text12"> <b> Original part:</b> <a href="https://parts.igem.org/Part:BBa_I2032" id="pageLink">BBa_I2032</a>
 
                <br><b>Submitted by:</b> MIT
 
                <br><b>Designed by:</b> Bartholomew Canton
 
                <br><b>Rational behind improvements:</b> BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our N<i>ex</i>t <i>vivo</i> system we have Codon optimized for use in <i>E. coli</i> and attached a N-terminal hexahistidine tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21 DE3 gold cells by putting it under the control of a T7 promoter (<a href="https://parts.igem.org/Part:BBa_I719005" id="pageLink">BBa_I719005</a>), RBS (<a href="https://parts.igem.org/Part:BBa_B0034" id="pageLink">BBa_B0034</a>) and double terminator (<a href="https://parts.igem.org/Part:BBa_B0015" id="pageLink">BBa_B0015</a>).
 
  
 
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Revision as of 21:50, 30 October 2017


T7 RNA Polymerase optimized for expression in E. coli

T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in Escherichia coli and contains a N-terminal histidine tag. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features</p>


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1719
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 1496
    Illegal NgoMIV site found at 1943
  • 1000
    COMPATIBLE WITH RFC[1000]