Revision as of 21:09, 30 October 2017

T7 Promoter+RBS+MiAMP1

Figure 1. P_T7+RBS+MiAMP1+terminator

Introduction

      This peptide is from the MiAMP family. It is an anti-fungal peptide which inhibits the growth of a variety of fungi and has no activity against E.coli. The mechanism of the peptide is to bind specifically to beta-(1,3)-glucans in fungal cell walls through conserved polar and aromatic residues. NCTU_Formosa used it to be one of a training data for creating our anti-fungal peptide scoring card.

Experiment

      We put T7 promoter, RBS, and MiAMP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 250 ~ 500 b.p.. Its PCR product is around 500 ~ 750 b.p..

Figure 2. P_T7 + RBS + MiAMP1
The DNA sequence length of P_T7 + RBS + MiAMP1 is around 250 ~ 500 b.p.. The size of its PCR product should be close to 600 ~650 b.p..

Reference

McManus, A.M.; Nielsen, K.J.; Marcus, J.P.; Harrison, S.J.; Green, J.L.; Manners, J.M.; Craik, D.J. "MiAMP1, a novel protein from Macadamia integrifolia adopts a Greek key beta-barrel fold unique amongst plant antimicrobial proteins." J. Mol. Biol. 1999, 293, 629-638

Sequence and Features