Difference between revisions of "Part:BBa K2449004"
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Revision as of 21:06, 30 October 2017


cep94A controlled by LacI promoter

cep94A is a cellobiose phosphorylase, which efficiently phosphorylates the cellobiose at its β-linkage, resulting in the degradation of cellobiose to D-glucose and α-D-glucose-1-phosphate.

Exprestion of cep94A

There were conducted an assay to see the expression of the protein coded by Cen94A. It has a weight on 92,7 kDa, it is easely seen on the SDS-PAGE. This is on the high copi plasmid;pSB1C3.

T--SDU-Denmark--SDS-page_cep94A.jpg

It was further testet, if the protein could be expressed on a medium copi plasmid;pSB3K3

T--SDU-Denmark--SDS-page_cep94A_medium_and_high_plasmid.jpg

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T--SDU-Denmark--Cellubiose_kuvette.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 994
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1072
    Illegal NgoMIV site found at 2168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334