Difference between revisions of "Part:BBa K2449004"
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<b>Exprestion of cep94A</b>
 
<b>Exprestion of cep94A</b>
  
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There were conducted an assay to see the expression of the protein coded by Cen94A. It has a weight on 92,7 kDa, it is easely seen on the SDS-PAGE.  
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There were conducted an assay to see the expression of the protein coded by Cen94A. It has a weight on 92,7 kDa, it is easely seen on the SDS-PAGE. This is on the high copi plasmid;<partinfo>pSB1C3</partinfo>.
 
<center>
 
<center>
 
https://static.igem.org/mediawiki/2017/b/b2/T--SDU-Denmark--SDS-page_cep94A.jpg
 
https://static.igem.org/mediawiki/2017/b/b2/T--SDU-Denmark--SDS-page_cep94A.jpg
 
</center>
 
</center>
  
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It was further testet is it was abel to expres the protein on a <partinfo>pSB3K3</partinfo>
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It was further testet, if the protein could be expressed  on a medium copi plasmid;<partinfo>pSB3K3</partinfo>
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+
<center>
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−
 
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https://static.igem.org/mediawiki/2017/0/00/T--SDU-Denmark--SDS-page_cep94A_medium_and_high_plasmid.jpg
 
https://static.igem.org/mediawiki/2017/0/00/T--SDU-Denmark--SDS-page_cep94A_medium_and_high_plasmid.jpg
 +
</center>
  
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https://static.igem.org/mediawiki/2017/1/1f/T--SDU-Denmark--Cellubiose_kuvette.jpg
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<center>
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https://static.igem.org/mediawiki/2017/2/21/T--SDU-Denmark--SD_Growth_experiment.svg
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</center>
  
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<center>
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https://static.igem.org/mediawiki/2017/1/1f/T--SDU-Denmark--Cellubiose_kuvette.jpg
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</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:03, 30 October 2017


cep94A controlled by LacI promoter

cep94A is a cellobiose phosphorylase, which efficiently phosphorylates the cellobiose at its β-linkage, resulting in the degradation of cellobiose to D-glucose and α-D-glucose-1-phosphate.

Exprestion of cep94A

There were conducted an assay to see the expression of the protein coded by Cen94A. It has a weight on 92,7 kDa, it is easely seen on the SDS-PAGE. This is on the high copi plasmid;pSB1C3.

T--SDU-Denmark--SDS-page_cep94A.jpg

It was further testet, if the protein could be expressed on a medium copi plasmid;pSB3K3

T--SDU-Denmark--SDS-page_cep94A_medium_and_high_plasmid.jpg

https://static.igem.org/mediawiki/2017/2/21/T--SDU-Denmark--SD_Growth_experiment.svg

T--SDU-Denmark--Cellubiose_kuvette.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 994
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1072
    Illegal NgoMIV site found at 2168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334