Difference between revisions of "Part:BBa K2404000"

Latest revision as of 20:47, 30 October 2017

A Thyroid Stimulating Hormone antagonist without His-Tags

This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH).

TSH was first generated and characterised by [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558]. The protein includes two parts, the beta subunit from Thyrotropin (TSH) fused to a common alpha subunit shared by hormone glycoproteins. This is linked by a short CTD linker sequence. This TSH antagonist has been modified to remove glycosylation sites that exist on both subunits. As a result this antagonist binds to the TSH receptor but does not activate it.

TSH has been expressed in a murine expression system and shown to inhibit the auto-immune antibodies that cause in Graves' disease ([http://www.pnas.org/content/113/5/1244.full Saxena et al (2015). PNAS 113, 1244-129]).

We intend to use this TSH protein as a therapeutic treatment to treats hyperthyroidism in humans.

This part has been cloned ito pSB1C3 using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard. It is flanked by BsaI sites together 5' aatg and 3' gctt cloning sequences for subsequent cloning into a level 1 plasmid.

We also generated a version of TSH containing a C-terminal His tag BBa_K2404001

Sequence and Features