Difference between revisions of "Part:BBa K2404001"
 
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TSH has been expressed in a murine expression system and shown to inhibit the auto-immune antibodies that cause in Graves' disease ([http://www.pnas.org/content/113/5/1244.full Saxena et al (2015). PNAS 113, 1244-129]).  
 
TSH has been expressed in a murine expression system and shown to inhibit the auto-immune antibodies that cause in Graves' disease ([http://www.pnas.org/content/113/5/1244.full Saxena et al (2015). PNAS 113, 1244-129]).  
  
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We intend to use this TSH protein as a therapeutic treatment to treats hyperthyroidism in himans.  
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We intend to use this TSH protein as a therapeutic treatment to treats hyperthyroidism in humans.  
  
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This part has been cloned ito pSB1C3 using BsmBI and conforms to the Phytobrick standard. It is flanked by BsaI sites together 5' aatg and 3' gctt  cloning sequences for subsequent cloning into a level 1 plasmid.  
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This part has been cloned ito pSB1C3 using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard. It is flanked by BsaI sites together 5' aatg and 3' gctt  cloning sequences for subsequent cloning into a level 1 plasmid.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:31, 30 October 2017


A Thyroid Stimulating Hormone antagonist with His-Tags

This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH). This includes a 6xHis-tags to aid protein purification.

TSH was first generated and characterised by [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558]. The protein includes two parts, the beta subunit from Thyrotropin (TSH) fused to a common alpha subunit shared by hormone glycoproteins. This is linked by a short CTD linker sequence. This TSH antagonist has been modified to remove glycosylation sites that exist on both subunits. As a result this antagonist binds to the TSH receptor but does not activate it.

TSH has been expressed in a murine expression system and shown to inhibit the auto-immune antibodies that cause in Graves' disease ([http://www.pnas.org/content/113/5/1244.full Saxena et al (2015). PNAS 113, 1244-129]).

We intend to use this TSH protein as a therapeutic treatment to treats hyperthyroidism in humans.

This part has been cloned ito pSB1C3 using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard. It is flanked by BsaI sites together 5' aatg and 3' gctt cloning sequences for subsequent cloning into a level 1 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 795
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 827