Difference between revisions of "Part:BBa K2449004"
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<partinfo>BBa_K2449004 short</partinfo>
 
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cep94A  
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cep94A is a cellobiose phosphorylase, which efficiently phosphorylates the cellobiose at its β-linkage, resulting in the degradation of cellobiose to D-glucose and α-D-glucose-1-phosphate
  
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https://static.igem.org/mediawiki/2017/1/1f/T--SDU-Denmark--Cellubiose_kuvette.jpg
 
  
  
 
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===Usage and Biology===
 
===Usage and Biology===
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https://static.igem.org/mediawiki/2017/b/b2/T--SDU-Denmark--SDS-page_cep94A.jpg
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https://static.igem.org/mediawiki/2017/0/00/T--SDU-Denmark--SDS-page_cep94A_medium_and_high_plasmid.jpg
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https://static.igem.org/mediawiki/2017/1/1f/T--SDU-Denmark--Cellubiose_kuvette.jpg
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Revision as of 20:26, 30 October 2017


cep94A controlled by LacI promoter

cep94A is a cellobiose phosphorylase, which efficiently phosphorylates the cellobiose at its β-linkage, resulting in the degradation of cellobiose to D-glucose and α-D-glucose-1-phosphate


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 994
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1072
    Illegal NgoMIV site found at 2168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334