Difference between revisions of "Part:BBa K2262013"
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<h1>'''Introduction'''</h1> | <h1>'''Introduction'''</h1> | ||
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It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function. | It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function. | ||
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<p style="padding:1px;font-size:14px"><b>(1) Inhibition Zone </b></p> | <p style="padding:1px;font-size:14px"><b>(1) Inhibition Zone </b></p> | ||
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<p style="padding:1px;font-size:14px"><b>(2) Spore Germination </b></p> | <p style="padding:1px;font-size:14px"><b>(2) Spore Germination </b></p> | ||
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<p style="padding:1px;font-size:14px"><b>(3) Botany Experiment </b></p> | <p style="padding:1px;font-size:14px"><b>(3) Botany Experiment </b></p> | ||
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| + | <p style="padding:1px;font-size:16px"><b>2. Cloning </b></p> | ||
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We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p.. | We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p.. | ||
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| + | [[File:Agarose gel Pe-4.png|200px|thumb|center|'''Figure 5.''' P<sub>T7</sub> + RBS + Pe-4 <br> | ||
| + | The DNA sequence length of P<sub>T7</sub> + RBS + Pe-4 is around 100 ~ 200 b.p.. The size of its PCR product should be close to 450 ~ 500 b.p..]] | ||
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Revision as of 19:57, 30 October 2017
T7 Promoter+RBS+Pe-4
Introduction
It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function.
Experiment
1. Fungal Test
(1) Inhibition Zone
(2) Spore Germination
(3) Botany Experiment
2. Cloning
We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]