Difference between revisions of "Part:BBa K2286005"

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  To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.  
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  To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR.  
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The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that  
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PYK10-1457 is an excellent root-specific promoter.  
  
 
[[File:T--UESTC-China--PYK10-1457-RT-PCR.png|500px|thumb|center|'''Fig 1'''.RT-PCR of CKX3 activated by PYK10-1457.]]
 
[[File:T--UESTC-China--PYK10-1457-RT-PCR.png|500px|thumb|center|'''Fig 1'''.RT-PCR of CKX3 activated by PYK10-1457.]]

Revision as of 18:14, 30 October 2017


PYK10-1457：a seedling and root specific promoter

This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR.

The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.

Fig 1.RT-PCR of CKX3 activated by PYK10-1457.