Difference between revisions of "Part:BBa K2448023"

Revision as of 18:11, 30 October 2017

Universal Biosensing Chassis (UBC)

Usage and Biology

The Universal Biosensing Chassis (UBC) is the keystone of our project. It aims to provide an answer to the lack of rapid and reliable building methods for transcription-factor based biosensors. Biosensors rely on a basic theoretical principle: a certain concentration of a molecule of interest induces a proportional production of a fluorescent compound. Transcription-factor based biosensors allow the precise and cheap detection or quantification of various chemical compounds.

Using our composite biobrick, one will only need a suitable transcription factor, able to bind to the molecule of interest, and its related promoter. However sometimes, there is no transcription factor in the databases that matches what we are looking for. Here we can use indirect sensing or Sensing-Enabling Metabolic Pathways. Essentially, tools like [http://sensipath.micalis.fr Sensipath] search and design the enzymatic reactions necessary to transform your non-detectable molecule in a detectable one, therefore expanding the repertoire of molecules available to sensing.

Apart from its fast building design, the UBC has been engineered to be used in high throughput procedures such as screening. Indeed, we originally designed it for a new enzyme engineering process for D-psicose production, using a psicose biosensor to identify the enzyme mutants showing the best activity. In addition, by its design, the UBC allows fast cloning: depending on what is to be inserted into the UBC and one’s mastery of the Golden Gate Assembly, a functional biosensor can be obtained in less than a week.

For more information: http://2017.igem.org/Team:Evry_Paris-Saclay

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1033
Illegal NheI site found at 1056
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1005
Illegal XhoI site found at 62
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 128
Illegal AgeI site found at 251
Illegal AgeI site found at 2154
Illegal AgeI site found at 2266
1000
COMPATIBLE WITH RFC[1000]

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 Universal Biosensing Chassis (UBC)
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The Universal Biosensing Chassis (UBC) is the keystone of our project. It aims to provide an answer to the lack of rapid and reliable building methods for transcription-factor based biosensors. Biosensors rely on a basic theoretical principle: a certain concentration of a molecule of interest induces a proportional production of a fluorescent compound. Transcription-factor based biosensors allow the precise and cheap detection or quantification of various chemical compounds.
+Using our composite biobrick, one will only need a suitable transcription factor, able to bind to the molecule of interest, and its related promoter. However sometimes, there is no transcription factor in the databases that matches what we are looking for. Here we can use indirect sensing or Sensing-Enabling Metabolic Pathways. Essentially, tools like [http://sensipath.micalis.fr Sensipath] search and design the enzymatic reactions necessary to transform your non-detectable molecule in a detectable one, therefore expanding the repertoire of molecules available to sensing.
+Apart from its fast building design, the UBC has been engineered to be used in high throughput procedures such as screening. Indeed, we originally designed it for a new enzyme engineering process for D-psicose production, using a psicose biosensor to identify the enzyme mutants showing the best activity. In addition, by its design, the UBC allows fast cloning: depending on what is to be inserted into the UBC and one’s mastery of the Golden Gate Assembly, a functional biosensor can be obtained in less than a week.
+For more information: http://2017.igem.org/Team:Evry_Paris-Saclay
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