Difference between revisions of "Part:BBa K2286012"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2286012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2286012 SequenceAndFeatures</partinfo>
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===Characterization===
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==Molecular weight==
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This halohydrin dehalogenase gene codes for a protein of 762 amino acids with a molecular mass of 29kDa[2].
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[[File:T--UESTC-China--DhaA31-RT-PC.png|500px|thumb|center|'''Fig 5'''.RT-PCR of positive plants.]]
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Revision as of 17:51, 30 October 2017


HheC: halohydrin dehalogenase

This is a halohydrin dehalogenase from Agrobacterium radiobacterAD1(HheC). HheC is an important enzyme in the halide dehalogenation reaction, which catalyzes o-halide to epoxides and hydrogen halides through intramolecular nucleophilic substitution mechanism. So it can catalyze many substrates, such as 1,3-dichloro propanol (2,3-DCP), 2,3-dichloropropane-1-ol (2,3-DCP), 3-chloropropane-1,2-diol (CPD) and so on.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Molecular weight

This halohydrin dehalogenase gene codes for a protein of 762 amino acids with a molecular mass of 29kDa[2].

File:T--UESTC-China--DhaA31-RT-PC.png
Fig 5.RT-PCR of positive plants.