Difference between revisions of "Part:BBa K2324013"
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<partinfo>BBa_K2324013 short</partinfo> | <partinfo>BBa_K2324013 short</partinfo> | ||
− | This part synthesises a FimH adhesin protein fused with a metallothionein at | + | This part synthesises a FimH adhesin protein fused with a metallothionein at the first amino acid position. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a metal binding FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the <i>fim operon</i> . |
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Revision as of 17:42, 30 October 2017
pRha_FimH_1His
This part synthesises a FimH adhesin protein fused with a metallothionein at the first amino acid position. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a metal binding FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 469
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 612