Difference between revisions of "Part:BBa K2262001"
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<h1>'''Introduction'''</h1> | <h1>'''Introduction'''</h1> | ||
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This peptide is from the MiAMP family. It is an anti-fungal peptide which inhibits the growth of a variety of fungi and has no activity against E.coli. The mechanism of the peptide is to bind specifically to beta-(1,3)-glucans in fungal cell walls through conserved polar and aromatic residues. NCTU_Formosa used it to be one of a training data for creating our anti-fungal peptide scoring card. | This peptide is from the MiAMP family. It is an anti-fungal peptide which inhibits the growth of a variety of fungi and has no activity against E.coli. The mechanism of the peptide is to bind specifically to beta-(1,3)-glucans in fungal cell walls through conserved polar and aromatic residues. NCTU_Formosa used it to be one of a training data for creating our anti-fungal peptide scoring card. | ||
<h1>'''Experiment'''</h1> | <h1>'''Experiment'''</h1> | ||
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We put T7 promoter, RBS, and MiAMP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 250 ~ 500 b.p.. Its PCR product is around 500 ~ 750 b.p.. | We put T7 promoter, RBS, and MiAMP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 250 ~ 500 b.p.. Its PCR product is around 500 ~ 750 b.p.. | ||
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| + | [[File:Agarose gel MiAMP1.png|400px|thumb|center|'''Figure 2.''' P<sub>T7</sub> + RBS + MiAMP1 <br> | ||
| + | The DNA sequence length of P<sub>T7</sub> + RBS + MiAMP1 is around 250 ~ 500 b.p.. The size of its PCR product should be close to 600 ~650 b.p..]] | ||
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<h1>'''Reference'''</h1> | <h1>'''Reference'''</h1> | ||
Revision as of 17:37, 30 October 2017
T7 Promoter+RBS+MiAMP1
Introduction
This peptide is from the MiAMP family. It is an anti-fungal peptide which inhibits the growth of a variety of fungi and has no activity against E.coli. The mechanism of the peptide is to bind specifically to beta-(1,3)-glucans in fungal cell walls through conserved polar and aromatic residues. NCTU_Formosa used it to be one of a training data for creating our anti-fungal peptide scoring card.
Experiment
We put T7 promoter, RBS, and MiAMP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 250 ~ 500 b.p.. Its PCR product is around 500 ~ 750 b.p..
Reference
McManus, A.M.; Nielsen, K.J.; Marcus, J.P.; Harrison, S.J.; Green, J.L.; Manners, J.M.; Craik, D.J. "MiAMP1, a novel protein from Macadamia integrifolia adopts a Greek key beta-barrel fold unique amongst plant antimicrobial proteins." J. Mol. Biol. 1999, 293, 629-638
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]