Difference between revisions of "Part:BBa K1119008"

Latest revision as of 17:07, 30 October 2017

CMV promoter - GFP - hGH polyA tail

This is a mammalian GFP generator used for characterization of our CMV promoter (BBa_K1119006). This GFP generator contains the CMV promoter sequence with GFP reporter in RFC25 format(BBa_K648013) and hGH polyA terminator (BBa_K404108).

This GFP generator was then transfected into HEK293FT cells and in vivo green fluorescence signal was observed under confocal microscope.

pEGFP-N1 (Clontech) served as the positive control. It contains the CMV promoter and EGFP reporter. A GFP generator that does not contain the CMV promoter was used as the negative control.

The [http://2013.igem.org/Team:Hong_Kong_HKUST/characterization detailed protocol] of our characterization can be found in HKUST iGEM 2013 Wiki.

Figure 1. CMV promoter drives expression of GFP. HEK cells transfected with pCMV-GFP gave GFP signals. HEK cells transfected with the commercial pEGFP-N1 showed similar results, while the same construct without any promoter did not give any GFP signals.

Improved Part

iGEM Technion 2017 improved this part to BBa_K2520006. This improved device contains a TRE promoter instead of the original CMV, to allow for inducible expression of GFP within mammalian cells.

Sequence and Features