Difference between revisions of "Part:BBa K2505000"
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<partinfo>BBa_K2505000 parameters</partinfo> | <partinfo>BBa_K2505000 parameters</partinfo> | ||
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| − | This gene is derived from <i>Arabidopais thaliana</i> and encode a receptor of | + | This gene is derived from <i>Arabidopais thaliana</i> and encode a receptor of cytokinins. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AHK4 has a histidine kinase activity, and binding of a cytokinin to AHK4 triggers auto-phosphorylation of AHK4 and the following histidine-to-aspartate phosphorelay. As a consequence, transcription from target genes is induced and/or repressed so that physiological states of plants are changed. |
The DNA sequences of this gene is optimized for expressing in <i>E. coli</i> cells considering the codon usage. | The DNA sequences of this gene is optimized for expressing in <i>E. coli</i> cells considering the codon usage. | ||
Revision as of 16:44, 30 October 2017
rbs-ahk4
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 76
Illegal BamHI site found at 1157 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 324
Illegal SapI.rc site found at 1959
This gene is derived from Arabidopais thaliana and encode a receptor of cytokinins. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AHK4 has a histidine kinase activity, and binding of a cytokinin to AHK4 triggers auto-phosphorylation of AHK4 and the following histidine-to-aspartate phosphorelay. As a consequence, transcription from target genes is induced and/or repressed so that physiological states of plants are changed. The DNA sequences of this gene is optimized for expressing in E. coli cells considering the codon usage.
Characterization
We tested the function of AHK4 protein that receives iP and induces histidine kinase phosphorylation in E.coli cell. Our objective was to characterize the function of AHK4 protein.