Difference between revisions of "Part:BBa I723021:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_I723136 short</partinfo>
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<partinfo>BBa_I723021 short</partinfo>
  
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<partinfo>BBa_I723136 SequenceAndFeatures</partinfo>
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<partinfo>BBa_I723021 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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[Christine/Maia]
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This system contains a PstI site and needs site directed mutagenesis. It requires the presence of the responsive promoter Pu primary to allow exprssion of a desired gene after exposure to BTEX compounds
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+
  
 
===Source===
 
===Source===
  
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[Christine/Maia]
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Cloned from the TOL plasmid from ''Pseudomonas putida'' MT2. The terminators were added separately.
  
 
===References===
 
===References===
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Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by ''Pseudomonas putida'' (''arvilla'') MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.

Latest revision as of 17:13, 25 October 2007


XylR transcriptional regulator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 530
    Illegal BglII site found at 1992
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1183


Design Notes

This system contains a PstI site and needs site directed mutagenesis. It requires the presence of the responsive promoter Pu primary to allow exprssion of a desired gene after exposure to BTEX compounds

Source

Cloned from the TOL plasmid from Pseudomonas putida MT2. The terminators were added separately.

References

Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.