Difference between revisions of "Part:BBa K2407314"

 
 
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<partinfo>BBa_K2407314 short</partinfo>
 
<partinfo>BBa_K2407314 short</partinfo>
  
TDH3+VOX+RFP+Adh1T+Ura3T+VOX+crtE
+
==Introduction==
 +
We use this composite to compare the phenomenon before and after mating. Initially, the Red Fluorescence Protein expresses. When the vika-vox system begin to work, the component between two vox sites will be deleted and the β-carotene gene will synthesize β-carotene. Yeasts turns from red to orange.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:03, 30 October 2017


TDH3 + vox + RFP + Adh1T + Ura3T + vox + β-carotene

Introduction

We use this composite to compare the phenomenon before and after mating. Initially, the Red Fluorescence Protein expresses. When the vika-vox system begin to work, the component between two vox sites will be deleted and the β-carotene gene will synthesize β-carotene. Yeasts turns from red to orange.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2653
    Illegal BglII site found at 4340
    Illegal XhoI site found at 2352
    Illegal XhoI site found at 2484
    Illegal XhoI site found at 3303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5244
    Illegal BsaI site found at 7599
    Illegal BsaI.rc site found at 2080
    Illegal BsaI.rc site found at 7930
    Illegal SapI site found at 4644