Difference between revisions of "Part:BBa K2507008"
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<partinfo>BBa_K2507008 short</partinfo>
 
<partinfo>BBa_K2507008 short</partinfo>
  
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== Background ==
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==Usage and Biology==
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E.coli codon optimized ThsS(BBa_K2507000) and ThsR(BBa_K2507001) are two basic parts which belong to the two-component system from marine Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell and ThsR is the DNA-binding response regulator(RR). PphsA(BBa_K2507018) is a ThsR activated promoter when ThsR is phosphorylated by ThsS after ThsS sensing thiosulfate.
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Because of thiosulfate is an indicator of gut inflammation (Levitt et al, 1999; Jackson et al, 2012; Vitvitsky et al, 2015), this system can be used as a sensor of gut inflammation.
  
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===Usage and Biology===
  
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This part contain a promoter J23105, ThsR coding which can response to the result of BBa_2507012 which contain a ThsS coding, promoter PphsA342 and sfGFP coding.
 
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ThsR (BBa_K2507003) is the report part of the thiosulfate sensor which belong to the ThsS/R two-component system. This system is from S. typhimurium (Hensel et al, 1999; Price-Carter et al, 2001).
 
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Combining with BBa_2507012 which contain the ThsS coding, those two part can  produce GFP after they sense the existence of thiosulfate.

 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2507008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2507008 SequenceAndFeatures</partinfo>
  
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==Characterization==
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fdgljsk
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==Reference==
  
 
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<partinfo>BBa_K2507008 parameters</partinfo>
 
<partinfo>BBa_K2507008 parameters</partinfo>
 
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<h2 id="results">Results</h2>
 
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Revision as of 15:55, 30 October 2017


J23105-thsR-PphsA342-sfGFP

Usage and Biology

E.coli codon optimized ThsS(BBa_K2507000) and ThsR(BBa_K2507001) are two basic parts which belong to the two-component system from marine Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell and ThsR is the DNA-binding response regulator(RR). PphsA(BBa_K2507018) is a ThsR activated promoter when ThsR is phosphorylated by ThsS after ThsS sensing thiosulfate. Because of thiosulfate is an indicator of gut inflammation (Levitt et al, 1999; Jackson et al, 2012; Vitvitsky et al, 2015), this system can be used as a sensor of gut inflammation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1313

Characterization

fdgljsk

Reference