Difference between revisions of "Part:BBa K2348000:Design"
(→‎References)
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===References===
 
===References===
 +
<sup>1</sup>Bingham, R. J., Hall, K. S. & Slonczewski, J. L. Alkaline induction of a novel gene locus, alx, in
 +
Escherichia coli. Journal of bacteriology 172, 2184–2186 (1990).
 +
 +
<sup>2</sup>Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A., Westhof, E. & Altuvia, S. A pH-responsive
 +
riboregulator. Genes & development 23, 2650–2662 (2009).

Revision as of 15:36, 30 October 2017


alx - alkaline regulated riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 312


Design Notes

The alx regulatory part contains the constitutiv alx promoter and the riboswitch itself


Source

Colony PCR from E. coli BL21

References

1Bingham, R. J., Hall, K. S. & Slonczewski, J. L. Alkaline induction of a novel gene locus, alx, in Escherichia coli. Journal of bacteriology 172, 2184–2186 (1990).

2Nechooshtan, G., Elgrably-Weiss, M., Sheaffer, A., Westhof, E. & Altuvia, S. A pH-responsive riboregulator. Genes & development 23, 2650–2662 (2009).