Difference between revisions of "Part:BBa K2336036:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
In order to replace GFP more conveniently, we respectivey added HindIII and SalI at both ends. Also, to avoid cutting pET28a, we changed SalI into XhoI.
+
In order to replace GFP more conveniently, we respectivey added HindIII and SalI at both ends. Also, to avoid cutting pET28a, which may influence our construct. we changed SalI into XhoI.
  
 
===Source===
 
===Source===

Latest revision as of 15:16, 30 October 2017


PmrC-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 397
    Illegal XhoI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1085


Design Notes

In order to replace GFP more conveniently, we respectivey added HindIII and SalI at both ends. Also, to avoid cutting pET28a, which may influence our construct. we changed SalI into XhoI.

Source

Synthesized by IDT.

References