Difference between revisions of "Part:BBa K2336036:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | In order to replace GFP more conveniently, we respectivey added HindIII and SalI at both ends. Also, to avoid cutting pET28a, which may influence our construct. we changed SalI into XhoI. | |
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===Source=== | ===Source=== | ||
| − | + | Synthesized by IDT. | |
===References=== | ===References=== | ||
Latest revision as of 15:16, 30 October 2017
PmrC-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 397
Illegal XhoI site found at 436 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1162
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1085
Design Notes
In order to replace GFP more conveniently, we respectivey added HindIII and SalI at both ends. Also, to avoid cutting pET28a, which may influence our construct. we changed SalI into XhoI.
Source
Synthesized by IDT.